Roche Realtime PCR LightCycler® 480 II System +
Description of the Asset : Realtime PCR
The LightCycler® 480 System is a proven high-performance, medium- to high-throughput PCR platform that provides various methods for gene detection, gene expression analysis, genetic variation analysis, and array data validation. Cutting-edge thermal block and optical technology lead to excellent well-to-well homogeneity and maximized detection sensitivity. Generate meaningful results via the state-of-the-art data analysis methods, in line with MIQE guidelines, and analyze true raw data without the need for passive reference dyes or normalization plates. The LightCycler® 480 System features a versatile, plate-based, real-time PCR device that supports mono- or multicolor applications. LightCycler® 480 System and related software, kits, and probes to perform gene expression analysis, SNP genotyping, and mutation scanning via high resolution melting (HRM).
|Gene Quantification Gene detection Absolute quantification Relative quantification/gene expression analysis|
|Genetic Variation Analysis Melt-curve genotyping with HybProbe- or SimpleProbe probes Endpoint genotyping with hydrolysis probes Gene Scanning or Methylation analysis with High Resolution Melting (HRM)|
|Multiple Plate Analysis 96- or 384-well plates|
• Fast, highly sensitive, and reproducible results for qualitative and quantitative detection of nucleic acids, mutation scanning, and SNP analysis
• Basic and advanced methods for gene expression and genotyping based on endpoint analysis or melting curves.
• Wide range of probe formats and fluorescent dyes.
• White or clear multiwell plates to best fit your assay format, application, and workflow.
Date of Receiving the Asset : 1-7-2008
Asset Original Cost : 1672047
Purchase Order Number : 5-91/2007-08/5725/28.3.08
Invoice Number : 4060128303/21.5.08
Vendor Name : Roche diagnostics asia pacific pte ltd, Singapore
Location : Dr.B.R.Shome
Present Status : To be attended by service engineer to make it functional
Pulsefield gel electrophoresis +
Description of the Asset : Pulsed field electrophoresis is a technique for resolving chromosome-sized DNAs. By alternating the electric field between spatially distinct pairs of electrodes, megabase (mb) sized DNAs are able to reorient and move at different speeds through the pores in an agarose gel. The CHEF-DR III system separates large and small DNA fragments with better resolution, speed, and accuracy, than initial pulsed field methods. DNAs from 100 bases to over 10 megabases (mb) may be effectively resolved. Pulsed field electrophoresis is a technique for resolving chromosome-sized DNAs. By alternating the electric field between spatially distinct pairs of electrodes, megabase (mb) sized DNAs are able to reorient and move at different speeds through the pores in an agarose gel. The CHEF-DR III system separates large and small DNA fragments with better resolution, speed, and accuracy, than initial pulsed field methods. DNAs from 100 bases to over 10 megabases (mb) may be effectively resolved. The CHEF-DR III system uses two leading technologies, CHEF (Clamped Homogeneous Electric Fields) and PACE (Programmable Autonomously Controlled Electrodes). The system provides highly uniform, or homogeneous, electric fields within the gel, using an array of 24 electrodes, which are “clamped” or held to intermediate potentials to eliminate lane distortion. Thus, lanes are straight. The system maintains uniform fields using patented Dynamic Regulation. The electrodes sense changes in local buffer conductivity due to buffer breakdown, change in buffer type, gel thickness, or temperature, and poten1 tials are readjusted immediately to maintain uniform fields, thus insuring high resolution. In PACE, the voltage potential of each of the 24 electrodes is regulated independently. The CHEF-DR III system can generate field angles from 90–120°.
Date of Receiving the Asset : 20.4.2010
Asset Original Cost : 2004274
Purchase Order Number : 5-139/09.10/ 3993-98/9-3-10
Invoice Number : S10-10036094-13.4.2010
Vendor Name : Biorad pacific ltd, Hongkong
Location : Dr.RajeswariShome
Applications: Applications include top down and bottom up mapping, electrophoretic karyotyping, analysis of tumor cell DNA rearrangements, mammalian DNA analysis, and testing for bacterial, yeast, and parasite strain homogeneity
Molecular Imager system FluorChem Q from Alpha Innotech +
Description of the Asset : Molecular Imager system FluorChem Q from Alpha Innotech
Alpha Innotech designed for imaging and analysis of multi-color fluorescent Western blots in a quantitative manner. There is a dual wavelength UV transilluminator and a white light folding table for stained gels. The additional epi-illumination light sources include four light sources: epi UV, 475 nm blue, 534 nm green and 632 nm red lights. The LED excitation lights are more convenient for the imaging of agarose gels than a UV-transilluminator. Many common dyes (e.g. SybrGreen, SybrGold, SybrSafe) can be excited with the 475 nm blue light just as well as with a UV transilluminatior. The benefit is that the background is lower and the image quality is better compared to the UV excitation
Date of Receiving the Asset : 20-Apr-10
Asset Original Cost : 2026590
Purchase Order Number : 5-145/3169-74/8.3.10
Invoice Number : 0037595/1.4.10
Vendor Name : Cell bioscience, USA
Location : Dr.B.R.Shome
Application : Multi-color imaging provided by the instrument allows to simultaneously and independently detect two or even three different proteins, which saves a lot of time and improves the results.
Trinocular microscope model Eclips 80i +
“fly-eye” optics ensure uniform illumination, Plan Apo VC objectives deliver high-resolution images, right to the edge with excellent resolution and uniform brightness up to the edges. Excellent fluorescence imaging capabilities. The DS-2Mv-L2 digital camera is space-saving and enables easy imaging without a PC. The 80i has a bright illumination system with a 12v-100w halogen lamp and is with a noise terminator, which directs stray light away from the light-collection path, increasing image contrast and clarity. The Eclipse 80i delivers remarkably high signal to noise ratios, producing fluorescence images that reveal more than ever before Equipment to be centrally controlled, enabling advanced imaging of fluorescence images.
Make : Nikon
Model : Eclipse 80i with Fluorescence and digital camera.
Date of Receiving the Asset : 17-May-10
Asset Original Cost : 1432817
Purchase Order Number : 5-141/3234-39/10.3.10
Invoice Number : KKA2340/22.4.10
Vendor Name : Nikon corporation, Japan
Location : Dr.RajeswariShome
Applications : Used for brightfield and flurocence imaging.
For teaching purposes as the large number of students can wach live image/ video.
Ultracentrifuge with accessories +
Sorvall™ MTX 150 Micro-Ultracentrifuge
With good acceleration rate of 1,50,000 rpm which reaches in a short period of time. The range of RCF of 1,048, 000xg supports any separation. This benchtop ultracentrifuge offers powerful separation technology in a quiet, compact, easy to use benchtop design.
Rotor Type: S 52 ST-0319
Tubes/Buckets: 4 (5 ml capacity)
Description of the Asset : Ultracentrifuge with accessories
Date of Receiving the Asset : 21.6.2010
Asset Original Cost : 2207186
Purchase Order Number : 5-140/3200.05/22.3.2010
Invoice Number : 50617643/26-5-2010
Vendor Name : Thermo scientific electronic instruments, USA
Location : Dr.S.S.Patil
Description of the Asset : Cryostat
Date of Receiving the Asset : 27-May-11
Asset Original Cost : 1029455
Purchase Order Number : 5-202/2924-28/3.3.11
Invoice Number : 94686518/6.5.11
Vendor Name : Leica Mikrosystemsvertrieb GmbH, Germany
Location : Dr. P.Krishnamoorthy
Flow Cytometer (BD FACS canto II/4/2/2 system with integrated fluidics cart etc) +
Description of the Asset : The BD FACSCanto™ II system has the versatility to meet demanding research requirements. The system brings BD flow cytometry and multicolor analysis to a new level to help researchers meet complex challenges and advance their research. BD FACSDiva™ software efficiently controls the setup, acquisition, and analysis of flow cytometry data from the BD FACSCanto II workstation. BD FACSDiva software enables researchers to preview and record data from multiple samples with an automated acquisition process. Acquisition templates, user-defined experiment layouts, and simple compensation procedures also managed by the software further facilitate acquisition. For analysis, the software includes powerful features such as hierarchical snap-to gating, a variety of plots, and batch analysis. Recorded data can be analyzed by creating plots, gates, population hierarchies, and statistical views on a BD FACSDiva global worksheet. The global worksheet can be used to analyze multiple sample tubes from the same experiment, to save time. User-defined batch analysis and automated capabilities such as gate resizing, pausing between data files, exporting statistics, and printing before proceeding to the next data file are additional BD FACSDiva features.
Date of Receiving the Asset : 18-8-2010
Asset Original Cost : 5771357
Purchase Order Number : 6-2(31)/naip/ admas/09-10/719-24/15-7-2019
Invoice Number : 933115049/18-8-2010
Vendor Name : Becton Dickinson & Co., Singapore
Application: Fluorescent dye tagged antibody marker based sorting of immunocompetent cells.
Status : Needs service
Automated Microbial identification system with software & data station complete +
Description of the Asset : The 3500 Genetic Analyzer is an 8-capillary sequencing instrument specifically designed to support the demanding performance needs of validated and process controlled environments, that life science researchers expect.
This package includes an 8-capillary instrument, 3500 Series Data Collection Software, a Dell Workstation and monitor, as well as secondary analysis software. The 3500 Series Data Collection Software supports sequencing and fragment analysis, and integrates seamlessly with downstream software for secondary analysis of genetic data. This package includes such secondary software: GeneMapper® software for fragment analysis and software for resequencing applications.
• Single-line, 505 nm, solid-state, long-life laser that utilizes a standard power supply and requires no heat-removal ducting
• Powerful, integrated data collection and primary analysis software provides real-time assessment of data quality
• Radio frequency identification (RFID) technology tracks key consumables data and records administrative information
• Advanced multiplexing capabilities for DNA fragment analysis with up to six unique dyes
• Unrivaled application flexibility—one array and one polymer are used for most applications.
• Simple setup, operation and maintenance
Confidently Identify Microbes : The MicroSEQ® system comprises bacterial libraries of 500 bp 16S rDNA sequences and over 2300 validated entries--including extensive coverage of gram-negative nonfermenters, Bacillus, Coryneforms, Mycobacteria, and Staphylococcus. A fungal library containing more than 1100 entries is also included. To help ensure accurate identification of the species of sequences, MicroSEQ® libraries contain only correctly identified organisms and quality-controlled sequences derived from type strains of preeminent culture collections. The libraries also support sequence polymorphisms, and include bacterial Category A biothreat agents.
Date of Receiving the Asset : 29-3-2010
Asset Original Cost : 8136120
Purchase Order Number : 6-2(30)/naip/admas/09-10/2392-99/15-7-2009
Invoice Number : 2100801218/29-3-2010
Vendor Name : Applied Biosystem International Incorporated
Location : Dr.B.R.Shome
Application : rRNA gene sequence based identification of microbe
Status : Needs service
Horizontal double door autoclave +
Description of the Asset : Horizontal double door
Date of Receiving the Asset : 31-12-2014
Asset Original Cost : 11,45,000
Purchase Order Number : F.5-336/P&S/NIVEDI/14-15/2486-89 dt: 26-7-2014
Invoice Number : MEQ/1299 dt: 28-8-2014
Vendor Name : M/s Periclave, Mumbai
Location : Dr. R.Yogisharadhya
ArcGIS software +
ArcGIS is a geographic information system (GIS) for working with maps and geographic information. ArcGIS provides contextual tools for mapping and spatial reasoning
1. Spatial Analytics: Spatial analytics is the heart and soul of ArcGIS and used to find the best location, plan for smarter communities, and prepare or respond faster in crucial situations. Useful for powerful spatial and temporal prediction modelling
2. Mapping & Visualization: Maps helps to spot spatial patterns in the data so as to make better decisions and take action. Maps also break down barriers and facilitate collaboration. ArcGIS gives you the ability to create, use, and share maps on any device.
3. Imagery & Remote Sensing: ArcGIS powerful for spatial image management, process, analyse, and share imagery. Not only do we get access to the world's largest imagery collection, we also get tools like satellite, aerial, drone and full motion video.