Equipments & Facilities
--> Absolute quantification
--> Relative quantification
• Genetic variation analysis/Genotyping
(Genotyping based on endpoint analysis/melting curve analysis; Mutation scanning & SNP analysis through High resolution melting (HRM) analysis)
• Gene expression analysis
W 60 cm x D 60 cm x H 54.5 cm <br>
W 24 in x D 24 in x H 21.5 in<br>
• Weight: 55 kg (121 lbs)<br>
• Temperature control<br>
->Peltier-based heating/cooling from 40°C - 95°C<br>
->Heated lid, PCR without any overlay (e.g., wax or oil)<br>
->Passive post-run cooling to < 40°C<br>
• 5 excitation filters, high-intensity broad-spectrum xenon lamp<br>
• Run time: <br>
->60 minutes (96-well plate)<br>
40 minutes (384-well plate)<br>
• Gene Scanning or Methylation analysis with High Resolution Melting (HRM)
• Use the plate configuration box to determine sample number, row A1-12 is used for duplicate samples of standards and a no template control (NTC).
• Unknowns should either be run as duplicates or triplicates.
• After dispensing the reaction volume, seal plate with optical adhesive cover using manufacturer’s instructions.
• Briefly spin down plate at 1,000 rpm and now plate is ready to run.
• At end of run, analyze PCR products by electrophoresis to confirm the absence of non-specific products.
Usage charges : charges per hour excluding GST and consumables
Sl. No. University National Laboratories/R&D organization Industry
1 Rs.1000/- Rs.2500/- Rs.2500/-
Progress of DNA amplification during a Polymerase Chain Reaction (PCR) can be monitored in "real time" (RT-PCR) by measuring the release of fluorescent "flashes" during amplification. Reaction rates can be measured continuously, or determined at a fixed time-point during the exponential amplification phase. It aids the Melt-curve genotyping with HybProbe- or SimpleProbe probes and Endpoint genotyping with hydrolysis probes.
The system design combines a state-of-the-art thermal cycler, an advanced optical system with an LED excitation source, and complete data analysis software. The instrument has five optics modules and the scanning optics design delivers optimal separation between the dyes and between samples. The instrument provides a closed-tube PCR detection format that can be used with a variety of fluorescence detection chemistries .
Initial release:December 27, 1999; 18 years ago
Stable release:10.5.1 / June 29, 2017; 6 months ago
Operating system:Desktop: Windows 7 SP1 and later, Windows Server 2008 SP2 and later; Server (x64 only) additionally supports: Windows Server 2003 SP2 and later; RHEL 5 Update 7 and later, SLES 11 SP1 and later.
Type:Geographic information system
License:Proprietary commercial software
Bacterial libraries of 500 bp 16S rDNA sequences and over 2300 validated entries
A fungal library containing more than 1100 entries, Radio frequency identification (RFID) technology tracks key consumables data and records administrative information , Advanced multiplexing capabilities for DNA fragment analysis with up to six unique dyes , Provision of GeneMapper® software for fragment analysis, MicroSEQ® ID Microbial Identification Software
Operating System: Windows 7, Windows Vista, Windows XP
Identify the unknown organism.
Assess the accuracy of the identification.
Examine results for all matches.
Determine if a match was made against a validated (proprietary) library or a custom library.
View phylogenetic relationships between the unknown and the top matches in a phylogenetic tree., To construct a phylogenetic tree, first search libraries to add sequences to tree and then search projects to add specimens to tree., Click ‘Compute Tree’. The Tree Computation dialog box opens, then click OK., Click the species name as desired in the tree, and then click the second species name as your second selection to view the Genetic Distance % for the selected species in the tree diagram, While viewing the tree, zoom the tree diagram as needed and select ‘File Print’ to print the tree diagram
MicroSEQ® ID Microbial Identification Software allows easily identifying and classifying unidentified bacterial or fungal sequences by comparing them to a validated microbial library. This bundled version includes the bacterial and fungal sequence libraries comprising of over 2300 microbial 16S rDNA sequences.
TCell components are fluorescently labelled and then excited by the laser to emit light at varying wavelengths. The fluorescence can then be measured to determine the amount and type of cells present in a sample.
10 to 20µm in 2 µm increments
20 to 60µm in 5µm increments, Cryochamber temperature (Section cutting area) : 0 to -35ºC, Low profile blades and high profile blades usage : One holder is available to hold both blades but the high
profile blades require pressure plate and it has to be fitted
and unscrewed whenever required, Time required for cooling to -35ºC temperature : 4 hours, Defrost cycle : Automated, Touch screen : Yes
The cryostat works in the principal that at low temperatures the liquid or the embedding medium freezes and acts as embedding material, the tissue becomes hard and becomes ice block. The microtome knife is used to make tissue sections of desired thickness without processing the tissues and fresh tissues can be used.
Director, ICAR-NIVEDI, PB No. 6450, Yelahanka, Bengaluru-560064
Phone: 080-23093111 Fax: 080-23093222
Email: [email protected]
Dr.S.S. Patil, ICAR-NIVEDI, Bengaluru (080-23093137)
There are different techniques/methods used for separation of proteins/antigens/nucleic acids using different gradients. Continuous sucrose gradient is usually followed for separation of viral proteins/antigens. Different gradients of sucrose are prepared in tubes and concentrated viral proteins are layered over the gradient with good acceleration of RCF 1,048,000xg for 3 hrs. The separated proteins can be collected from particular specific gravity band.
The ergonomic binocular-eyepiece tube can be inclined at angles from 10° to 30° and the eyepieces can be extended up to 40mm.
This ensures an optimum eye point and comfortable viewing posture
The Eclipse 80i delivers remarkably high signal to noise ratios, producing fluorescence images. The noise terminator directs stray light away from the light-collection path, which helps in high signal-to-noise ratios and ultimately high contrast and clarity of images.
DAfter electrophoresis, fluorescent dye stained agarose gel is exposed to a UV light source. The fluorescent dye bound nucleic acid fluoresces and becomes visible.
Construction: Aluminum chassis
Weight: 10 kg, Maximum amps: 0.5 amperes , Allowable voltage gradients: 0.6–9 V/cm, in 0.1 V/cm increments