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You are here: Home | Technologies | Protein-G Based Indirect ELISA Kit for Bovine Brucellosis

Brucellosis in Indian livestock is responsible for a median loss of US $3.4 billion/annum (INR 22554 crores) and is a well-known zoonotic disease.
Veterinary institutions were procuring imported ELISA kits for brucellosis surveillance/diagnosis until 2012 due to the unavailability of indigenous kits in India.
The screening and diagnosis using imported ELISA kits results in increased testing cost/sample, which directly impacts the national exchequer.

Hence, research was initiated to develop an indirect ELISA to make it available locally at a much lower cost than imported kits.

India has a huge bovine population, and brucellosis is considered the second most important disease of economic importance after FMD.
To screen the huge bovine population against brucellosis, an ELISA diagnostic kit was standardized, evaluated, and validated.
The kit has 98% and 92% sensitivity and 95% and 98% specificity in cattle and buffaloes, respectively.
A patent has been obtained for the kit (Patent No. 335659 dated 20.04.2020).
A total of 298 kits were supplied to 60 veterinary institutions from 2012 to date with limited laboratory scaling facility.
ICAR –NIVEDI Protein-G based iELISA kits are very popular in the country and have facilitated brucellosis screening in 1.60 lakh bovines in various institutions.
The NIVEDI i-ELISA kit cost/sample testing is approximately Rs 35.10 (in duplicate samples) against Rs 178.26/sample by imported ELISA kits (in duplicate samples).